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| Assay Type | Sandwich-ELISA |
| Analyte | Nuclease |
| Format | 96T(8×12 strips) |
| Reactivity | / |
| Regulatory Status | RUO |
| Sensitivity | ＜25pg/mL |
| Standard Curve Range | 25 pg/mL-800 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination the residue of nuclease in biologicals. |
| Sample volume | 100 uL |
| ID | Components | Size |
| CRS031-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate(8×12 strips) |
| CRS031-C02 | Nuclease Standard | 5 μg |
| CRS031-C03 | Biotin-Anti-Nuclease Antibody | 20 μg |
| CRS031-C04 | Streptavidin-HRP | 50 μL |
| CRS031-C05 | 10×Washing Buffer | 50 mL |
| CRS031-C06 | 2×Dilution Buffer | 50 mL |
| CRS031-C07 | Substrate Solution | 12 mL |
| CRS031-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease in different culture supernatant with similar curve and sensitivity.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease from different culture supernatant and was free from the matrix effects.
resDetect™ GENIUS™ Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A031) can detect the nuclease from different manufacturers, a nuclease levels differ from manufacturer to manufacturer.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three Nuclease with different concentrations were tested to calculate the recovery rate.
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